IN BOVINES AND SOME PROPERTIES OF STAPHYLOCOCCAL ISOLATES
M. ARSHAD, G. MUHAMMAD1,
M. SIDDIQUE, M. ASHRAF AND H.A. KHAN
of Veterinary Microbiology, 1Department of clinical Medicine and
of Veterinary Science,
Milk samples of 100 clinically mastitic bovines (50 each from buffaloes and
cows) were processed for bacterial isolation. A total of 90 bacterial isolates,
including 33 of staphylococci and 57 of other bacterial species were recovered.
Of the 33 staphylococcal isolates, 23 were identified as
Staphylococcus aureus and 10 as
Staphylococcus epidermidis. All
Staphylococcus aureus isolates were positive for deoxyribonuclease and
mannitol fermentation test and produced ß-haemolysis on blood agar. All
Staphylococcus epidermidis isolates
were negative to these tests and failed to produce ß-haemolysis on blood agar
containing 5% sheep erythrocytes. Penicillin resistance was detected in
13(56.5%) isolates of Staphylococcus
aureus and 8(80%) isolates of
Staphylococcus epidermidis. Penicillinase was produced by 4 of the 13
penicillin resistant isolates of
Staphylococcus aureus and 2 of the 8 penicillin resistant
Staphylococcus epidermidis isolates.
Mastitis, staphylococci, biochemical properties.