EVALUATION OF SIX CAMEL BREEDS FOR HETEROZYGOCITY THROUGH RESTRICTION FRAGMENT LENGTH POLYMORPHISM
M. G. SHAH, M.
REISSMANN1, A. S.
Qureshi2 AND H. J. SCHWARTZ3
Department
of Anatomy and Histology,
Sindh
Agriculture
University,
TandoJam,
Pakistan;1Institute
of Animal Sciences, Humboldt-Universität zu
Berlin,
Germany;
2Department
of Veterinary Anatomy,
University
of
Agriculture
Faisalabad,
Pakistan;
3Department
of Livestock Ecology, Humboldt-Universität zu
Berlin,
Germany
Abstract
In the camel tyrosinase gene, a restriction site provoked by the T
variant was used in a special restriction fragment length polymorphism analysis
(PCR-RFLP) for genotyping of animals from six different Pakistani camel breeds (Marecha,
Dhatti, Larri, Kohi, Campbelpuri and Sakrai). For this purpose, four new primer
pairs were designed for sequencing the coding region of exon 1 of the tyrosinase
gene. PCR reactions were carried out in a total volume
of 25
ml using 100 ng genomic DNA to amplify a 474 bp fragment at 56°C. A SNP (T/C) at 200 bps was found and exploited with a
Dde I restriction enzyme that resulted in three different genotypes i.e.
TT, TC and CC in each studied camel breed. Significant differences in the
genotype frequency between the breeds were recorded. The Sakrai breed showed a
distinctly higher frequency of heterozygous animals compared to Marecha, Dhatti,
Larri and Kohi breeds. Our new designed primers could be used for genotype
screening of other camel breeds. However, for understanding the contribution of
tyrosinase gene and its antagonist i.e. agouti in the coat colour production,
complete sequence of the gene is imperative.
Key words:
Camel, genotyping, RFLP,
tyrosinase.
