Abstract   

    Twenty pooled good quality buffalo bull semen samples were used in this study. Each sample was divided into five aliquots (A to E). Aliquot A was used to serve as control. Seminal plasma was removed by centrifugation from aliquot C, D and E. whereas it was not removed after centrifugation from aliquot B. Aliquot D was washed simply with 2.9% sodium citrate solution and aliquot E twice. All treated and control aliquots were extended in milk-egg-yolk glycerol extender and were used for insemination to note effect on conception rate (CR). CR was checked 45 days after insemination. The poorest 38.27% CR was observed with semen sample B. The CRs of samples A (53.85%), C (58.90%) and D (64.20) differed non significantly (P<0.05) from each other. But CR of E (69.30%) differed significantly (P<0.05) from sample A. However CR of sample E differed non significantly with the CR of C and D. It may be concluded that removal of seminal plasma and washing (single/double) improves conception rate in buffaloes   

Key words: Buffalo bull semen, washing, conception rate, field conditions.