During the present survey, 100 buffaloes were examined for lice infestation at a private cattle farm, situated in Multan, Pakistan.  Ninety two percent buffaloes were infested with Haematopinus spp., 6% with Damalinia spp. and 2% with Linognathus spp. The relationship between sex of animal and different lice was also determined.  It was 94.1% in females for Haematopinus spp., 5.8% for Damalinia spp. and 2.35% for Linognathus spp., while in males it was 80, 6.66 and 0 percent, respectively.  The relationship between age and different species of lice was also studied and in case of Haematopinus spp., it was significantly (P<0.05) higher (100%) in age group of 41 to 100 months compared to 14.2% in age group of 21 to 40 months.  For Damalinia spp. it was 14% in the age group of 21 to 40 months, while it was 0% in age group of 1-20 months.  At total of 8.33% buffaloes were infested with Linognathus spp. in age group of 61-80 months.