The prevalence of lice with species identification was studied during April to November 2006 in cows and buffaloes. During this period, various farm houses of Quetta city were randomly visited for the collection of lice. A total 909 cows and 671 buffaloes were examined for the presence of lice. Out of these, 38.3% cows and 41.2% buffaloes gave positive results for different lice species. Only one lice species i. e Bovicola bovis (38.3%) was recorded from cows and two species belonging to genus Haematopinus i. e. H. quadrpertusis (23.5%) and H. eurysternus (17.7%) were encountered in buffaloes.  Lice seem to be host specific with B. bovis occur in cows, while both H. quadripertusus and H. eurysternus usually infest buffaloes.