This study was carried out to know the prevalence of brucellosis in Urmia, Iran. For this purpose, 338 milk samples were collected from 36 villages in two seasons (spring and autumn, 2008). In spring, 82 milk samples from 11 villages and in autumn, 256 milk samples from 25 villages were collected randomly. These samples were examined for Brucella abortus antibodies with milk ring test. From 82 milk samples collected during spring, 1 (1.22%) showed positive and 81 (98.78%) showed negative reaction. From 256 milk samples collected during autumn, 3 (1.17%) showed positive and 253(98.83%) showed negative reaction. It was concluded from the results that prevalence of brucellosis in cattle was low in this region.

Key words: Brucellosis, Cattle, Iran, Milk ring test, Prevalence