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Pak Vet J, 2015,
35(2):
248-250
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Quantitative Pathology of Canine
Cortico-Cerebellar Degeneration |
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C Bianco, C Bombardi, G
Gandini, A Gallucci, R Sirri, G Sarli and L Mandrioli* |
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Department of Veterinary
Medical Sciences, Alma Mater Studiorum University of Bologna, Via
Tolara di Sopra 50, 40064 Ozzano Emilia, Italy;
*Corresponding author:
luciana.mandrioli@unibo.it |
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Abstract
The aim of this work was to document the value of
morphometric analysis in quantifying neuronal loss in canine cerebellar
degenerative disease.
Measurements of the cerebellar cell populations
in a dog affected by severe cortico-cerebellar degeneration (CCD) were compared
with those of an age-matched control dog. Histological stainings (hematoxylin-eosin,
thionin, Bielschowsky) were performed; the granule cell density and Purkinje
cell linear density were quantified. GFAP immunohistochemistry was run to
quantify the stained area. Morphometric analysis in the pathologic dog showed a
significant decrease of Purkinje cell linear density (4.50/mm),
granule cells density (81.34/10000 µm2) and an increase of GFAP stained area (40.28%) in comparison to the age-matched dog (Purkinje cell linear density=12.74/mm, granule cell neuron density=95.53/10000 µm2
and GFAP stained area=34.68%).
The use of
morphometric techniques is suggested in order to enable comparison between
results obtained from different laboratories of pathology.
Key words:
Abiotrophy,
Cerebellum,
Dog, Histochemistry, Immunohistochemistry,
Morphometry
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ISSN 0253-8318 (Print) ISSN 2074-7764 (Online)
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