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Determination of Median Lethal Dose of Enrofloxacin Microemulsion in Mice
Xue-Feng Yang§, Dong-Yang Liu§, Qing-Gong Han, Ya-Wei Sun and Guo-Ying Fan*
College of Animal Science, Henan Institute of Science and Technology, Xinxiang, Henan Province, P.R. China
*Corresponding author:


As a novel formulation of enrofloxacin, the toxicity of enrofloxacin microemulsion was unknown. The present study was conducted to determine the median lethal dose (LD50) of enrofloxacin microemulsion by using the acute toxicity test in mice. Based on the dose range of the pretest, mice in the group receiving the enrofloxacin microemulsion were intragastrically administered the dose levels of 1320.0, 1056.0, 844.8, 675.84, 540.67 and 432.6 mg/kg of body weight, respectively. LD50 calculated by Bliss method was 740.08 mg/kg, and 95% confidence limit of LD50 was 647.11~844.87 mg/kg, which indicated enrofloxacin microemulsion could be labeled as the hazard category 4 according to GHS and be considered as a low toxicity drug.

Key words: Acute toxicity test, Enrofloxacin, Median lethal dose, Mice, Microemulsion


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)