Bovine Herpes Virus-1 cause of infectious balanoposthitis, vulvovaginitis and respiratory diseases. Research had been conducted to develop new technique in BHV-1 diagnostic method. Detection of BHV-1was conducted using immupero-xidase monolayer assay (IPMA) with specific monoclonal antibodies anti BHV-1 which were reacted with a secondary antibody labeled horseradishperoxidase. Positive reaction showed in brown color mainly in nucleus of Madin Darby Bovine Kidney cells and the negative showed transparent/colorless cells. The study demonstrate that IPMA was able to detect viral antigen BHV-1 in cell culture.

Key words: Bovine Herpes Virus-1, Immunoperoxidase monolayer assay