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Immunomodulatory Activity of Pinus radiata Extract against Coccidiosis in Broiler Chicken
Asghar Abbas1, Zafar Iqbal1, Rao Zahid Abbas*1, Muhammad Kasib Khan1and Junaid Ali Khan2
1Department of Parasitology, University of Agriculture Faisalabad, Pakistan
2Institute of Pharmacy, Physiology and Pharmacology, University of Agriculture Faisalabad, Pakistan
*Corresponding author:


Immunomodulatory activity of Pinus radiata extract (PRE) against experimental Eimeria infection in broiler chicks was evaluated. For in-vivo trials, 175 day-old broiler chicks were divided into five equal groups (A, B, C, D and E) containing 35 chicks in each group. At one week of age, the groups A, B and C were given orally with graded doses of PRE (100, 200 and 300 mg/kg of body weight, respectively) for three consecutive days. Group D served as positive control (Vitamin-E treated) and Group E served as negative control (PBS treated). At 14th day of age, all groups were infected orally with 60,000 sporulated oocysts of mixed Eimeria species. Cell mediated immune response was evaluated by Phytohemagglutinin-P, Concanavalin-A, Carbon clearance assay and Dinitrochlorobenzene tests. Humoral immune response was evaluated by microplate hemagglutination test using sheep red blood cells. Results of study demonstrated that a dose dependent immune response was observed in PRE treated groups. Cell meditated and humoral immune response of group treated with PRE @ 300 mg/kg of body weight was comparable to positive control group (Vitamin-E treated) (P˃0.05). Pinus radiata extract treated groups showed significantly higher cell mediated and humoral response as compared to negative control (PBS) (P<0.05).

Key words: Coccidiosis, Eimeria, Immunity, Pinus radiata extract


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)