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Pak Vet J,
2020,
40(3):
283-288
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Effect of
Ethanol Extract and
Fractions of Physalis Calyx Seu Fructus
on Inflammation and
Mycoplasma
Gallisepticum |
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Yanyan Yi1,
Miaomiao Yao1, Panpan Sun2, Jia Cheng1,
Ajab Khan1, Jianhua Guo3, Hongquan Li1*
and Na Sun1* |
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1College
of Animal Science and Veterinary Medicine, Shanxi Agricultural
University, Taigu, Shanxi 030801, People’s Republic of China; 2Laboratory
Animal Center, Shanxi Agricultural University, Taigu, Shanxi 030801,
People’s Republic of China; 3Department of Veterinary
Pathobiology, Schubot Exotic Bird Health Center, Texas A&M
University, College Station, TX 77843, USA;
*Corresponding author:
snzh060511@126.com |
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Abstract
The
aim of the study was
to investigate whether ethanol extract and fractions from D-101 macroporous
resin of Physalis Calyx Seu Fructus has
the anti-inflammatory and anti-Mycoplasma gallisepticum (MG)
activity in vitro. Extract (P) was isolated from the dried calyces of
Physalis Calyx Seu Fructus with
80% ethanol under reflux. The five major fractions (P1, P2, P3, P4 and P5) were
obtained with ethanol gradient elution from D-101 macroporous resin column
loaded with P. P and Fraction P2 was enriched with luteoloside content, with
1.39% and 2.548%, respectively. P3 had significantly inhibited the expression of
IL-6, TNF-α and NO both at
protein and gene level in
lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages by the qRT-PCR analysis and ELISA assay. Moreover, P2,
P3, and P4 at its minimum inhibitory concentration inhibited the growth of MG.
Conclusively, the present study suggested that P3 is a potential agent against
MG and inflammation.
To Cite This Article:
Yi Y, Yao M, Sun P,
Cheng J, Khan A, Guo J, Li H and Sun N,
2020. Effect of ethanol extract and fractions of physalis calyx seu fructus on
inflammation and Mycoplasma
Gallisepticum. Pak Vet J, 40(3): 283-288.
http://dx.doi.org/10.29261/pakvetj/2020.036
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ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)
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