The liver is the largest metabolic organ performing metabolic, hematological functions and detoxifying toxins. Liver toxicity is the major outcome of environmental and metabolic toxins. HPLC analysis were conducted on Opuntia Ficus Indica (OFI) seed powder and fruit powder (FP) analyzed the total phenolic, total flavonoid, and quantified the nutritional composition, mineral composition, vitamin E, vitamin C as well the level of antioxidants. The efficacy study was closed labeled and consisted of twenty-eight days. The study was designed to analyze the antioxidant-dependent anti-inflammatory efficacy of Opuntia Ficus Indica (OFI) fruit powder (FP) and Opuntia Ficus Indica (OFI) seed powder (SP) against carbon tetra chloride (CCl₄) induced acute liver injury in Wistar rats. The first negative control group (n=10) was fed on a standard diet and the second positive control group (n=10) after liver injury through induction of carbon tetrachloride (CCl4) with the normal standard diet. Treatment groups received fruit powder (FP) and seed powder (SP) after induction of CCL4. Baseline parameters including liver functioning test, ALP, ALT and serum bilirubin were analyzed and study results revealed that the liver enzymes such as elevated ALP, ALT, and serum bilirubin were significantly (P<0.05) resolved in all treatment groups compared with control positive group. Oxidative stress markers were significantly improved in all treatment groups compared with the control group such as total antioxidant capacity was increased, and total oxidative stress was reduced in the blood serum of all treatment group fruit powder (FP), seed powder (SP) compared to the control group.