This study aimed to detect Feline Coronavirus (FCoV) in clinically suspected Feline Infectious Peritonitis (FIP) cases using RT-PCR and to perform phylogenetic analysis of the S gene. A total of 30 samples from 23 symptomatic cats were collected from Tangerang and Bogor, West Java, Indonesia. Sample types included ascites, pleural effusion, rectal swabs, plasma, and feces. FCoV detection was performed using RT-PCR targeting the 3′ UTR and S genes, followed by sequencing. Nineteen samples tested positive for FCoV, and 17 isolates from Tangerang were successfully sequenced. Pairwise homology of the local isolates of the partial S gene showed nucleotide similarities ranging from 88.45% to 100%, with most isolates exhibiting the highest identity (88.72%–91.68%) to an Indian FCoV strain (PP254209). All isolates exhibited adenine (A) at position 23,531, consistent with the wild-type enteric strain that resemble sequences commonly found in FECV. Phylogenetic analysis confirmed that all local FCoV isolates belong to the dominant Genotype I strain, forming a distinct region-specific monophyletic sub-lineage in Tangerang, Indonesia.

To Cite This Article: Poetri ON, Nadila R, Hidayat R, Jesifa LJ, Alfin ZWK, Handharyani E, Mayasari NLPI, Rahmi VA and Rizaldi DA, 2026. Molecular characterization and phylogenetic analysis reveal a distinct genotype i fcov sub-lineage in tangerang, west java, indonesia. Pak Vet J, 46(4): 1047-1050. http://dx.doi.org/10.29261/pakvetj/2026.077