PAKISTAN VETERINARY JOURNAL

Pak Vet J, xxxx, xx(x) xxx-xxx

Effect of Graded Levels of Dietary L- Methionine on Growth Performance, Body Composition, Amino Acid Requirements, Serum Biochemical Parameters and Gut Microbiota in Coho Salmon (Oncorhynchus Kisutch) Alevins Cultured in Freshwater

Hairui Yu^1*, Abdur Rahman^1,2,3,*, Leyong Yu⁴ , Govindhrajan Sattanathan^1,2, Lingyao Li^{1 ,5}, Muhammad Younus^6*, Muhammad Waqas⁷, Asghar Abbas⁸, Hafiz Muhammad Ishaq⁸, Xiaojing Wu^1,9

¹Key Laboratory of Biochemistry and Molecular Biology in Universities of Shandong (Weifang University), Weifang Key Laboratory of Coho Salmon Culturing Facility Engineering, Institute of Modern Facility Fisheries, College of Biology and Oceanography, Weifang University, Weifang 261061, China; ²Weifang Key Laboratory of Salmon and Trout Health Culture, Conqueren Leading Fresh Science and Technology Inc., Ltd., Weifang 261205, China; ³Department of Animal Sciences, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan; ⁴College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China; ⁵Shandong Collaborative Innovation Center of Coho Salmon Health Culture Engineering Technology, Shandong Conqueren Marine Technology Co., Ltd., Weifang 261108, China; ⁶Department of Pathology, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan; ⁷Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, Ondokuz Mayıs University, Atakum, 55139, Samsun, Türkiye; ⁸Department of Pathobiology and Biomedical Sciences, Muhammad Nawaz Sharif University of Agriculture Multan; ⁹College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China

*Corresponding author: yhr6003@hotmail.com, abdurrehman@uvas.edu.pk, younusrana@uvas.edu.pk

Abstract

This study aimed to evaluate the methionine requirement of coho salmon (Oncorhynchus kisutch) alevins. A total of 1,800 alevins (initial body weight: 0.338±0.002 g) were used in the experiment, distributed across six dietary treatments with three replicates per treatment and 100 alevins per replicate. Six dietary concentrations levels of L-methionine were as follows: 1.09%, 1.64%, 2.15%, 2.63%, 3.14%, and 3.67% of the feed, or 1.98%, 3.01 %, 3.91%, 4.75%, 5.74% and 6.66% of the feed protein. ANOVA test was applied to statistically analyze the data. The results of the study revealed that body weight and specific growth rate were higher at 2.15% methionine level. While body protein increased with the increase of methionine level in the feed, reaching the highest (P<0.05) level at 2.63 % methionine, the arginine content was significantly lower at 1.09% and 1.64% levels; the lysine and methionine content was significantly higher at 2.15% and 2.63% methionine levels. Body fat, ash content, and essential amino acid requirements were nonsignificant. Significantly higher ALT, aspartate AST, Lactobacillus, and Bacillus, and lower (P<0.05) total cholesterol and triglyceride, Coliform, and Vibrio were recorded in 2.15% and 2.63% L-Methionine group. It was concluded that dietary L-methionine levels of 2.15% and 2.63% effectively enhanced performance, body composition, serum biochemical parameters, and gut microbiota in coho salmon alevins. Furthermore, the quadratic polynomial model analysis indicated that a methionine requirement between 2.55% and 2.69% is optimal for achieving the best results in specific growth rate, feed conversion ratio, protein efficiency ratio, and body protein deposition.

To Cite This Article: Yu H, Rahman A, Yu L, Sattanathan G, Li L, Younus M, Waqas M, Abbas A, Ishaq HM and Wu X, 2025. Effect of Graded Levels of Dietary L- Methionine on Growth Performance, Body Composition, Amino Acid Requirements, Serum Biochemical Parameters and Gut Microbiota in Coho Salmon (Oncorhynchus Kisutch) Alevins Cultured in Freshwater. Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2025.155

Abstract

ISSN 0253-8318 (Print)ISSN 2074-7764 (Online)

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)