The objective of this study was to explore the effect of Fecal microbiota transplantation (FMT) on restoring antibiotic-induced gut microbiota dysbiosis and ameliorating endotoxemia in chickens, as well as its impact on chicken intestinal morphology and meat quality. Although FMT has been extensively investigated in mammals, its application in poultry, which serves as a major global food source species with unique physiological characteristics, remains insufficiently studied. Thirty white feather broilers were randomly divided into three groups: The control group was fed a basal diet (Control group), the basal diet supplemented with antibiotics (Antibiotic group), and the antibiotic treatment followed by fecal microbiota transplantation (Antibiotic+FMT group). Compared to the Antibiotic group, the 16S rDNA sequencing revealed a significant recovery of microbial community in the Antibiotic+FMT group (P<0.05), the morphological analysis showed that the Antibiotic+FMT group significantly increased the height of small intestinal villi and the number of small intestinal glands (P<0.05), the serum biochemical analysis indicated that the levels of TNF-α, IL-6, and LPS decreased significantly, while IL-10 increased significantly in the Antibiotic+FMT group (P<0.05). Additionally, FMT also significantly enhanced meat quality parameters (color and pH) (P<0.05). It is concluded that FMT can significantly enhance the diversity of gut microbiota and support the restoration of intestinal structural damage, thereby ameliorating endotoxemia in chickens, and improving chicken meat quality.

To Cite This Article: Chong X, Yang B, Qiu J, Chu M, Liang Y, Cheng W, Zhang H, Wang Y, Si M, Chen X and Qi Y and Wang H, 2025. Efficacy of fecal microbiota transplantation restores antibiotic-induced gut microbiota dysbiosis and ameliorates endotoxemia of chickens. Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2025.289