Diabetic wound healing remains a major clinical challenge, necessitating the development of more effective therapies. In our study, we explored the potential of topically applied, chemically synthesized nanoparticles (NPs) as a novel therapeutic strategy for diabetic wound healing. This study aimed to evaluate the efficacy of these NPs based ointments on diabetic wound healing through tissue regeneration, using streptozotocin-induced diabetic albino rats. For this purpose, thirty-six male albino rats were divided into six groups, T0 (Control Normal, CN), T0 (Diabetic Control, DC), T1 (treated with AgNPs @3mg/ml), T2 (treated with CuNPs @2mg/ml), T3 (treated with ZnNPs @5mg/ml), and T4 (treated with Ag/Cu/Zn nanocomposite @3.5mg/ml). The diabetes was induced by intraperitoneal injection of streptozotocin (40mg/kg body weight) in experimental rats. After that, the 10mm diabetic wound was induced on the dorsal lumber region of each rat by surgical blade and treated with AgNPs, CuNPs, ZnNPs, and Ag/Cu/Zn nanocomposite-based ointments. The results suggested significant improvements (P<0.05) in wound contraction rate and healing time in the T4 group treated with Ag/Cu/Zn nanocomposite ointment. Furthermore, histological analysis showed a significant (P<0.01) effect on the formation of new epithelium, granulation tissue, angiogenesis, and collagen deposition in the T4 group treated with Ag/Cu/Zn nanocomposite ointment, resulting in diabetic wound regeneration. In conclusion, the Ag/Cu/Zn nanocomposite ointment not only promotes diabetic wound healing but also significantly improves the structural integrity of the regenerated tissue, highlighting its potential as an effective therapeutic option for diabetic wounds.

To Cite This Article: Murtaza G, Kausar R, Sial S, Hameed A, Rehan S, Kanwal A and Sharif MU, 2025. Targeting diabetic wound healing through tissue regeneration with silver, copper, and zinc nanoparticles in streptozotocin-induced diabetic albino rat model. Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2025.223