PAKISTAN VETERINARY JOURNAL

Pak Vet J, xxxx, xx(x) xxx-xxx

Comparison of the Anti-tumoral Effects of Kefir with Buffalo and Cow Yogurt on 1,2-Dimethylhydrazine-induced Colon Cancer in Mice

Ibrahim Demirkan¹, Tolga Ertekin², Musa Korkmaz¹, Sebnem Pamuk³, Hayati Yuksel⁴, Mehmet Fatih Bozkurt⁵, Mehmet Ozdemir⁶, Muhammad Nasir Bhaya^5,7*, Aysun Cevik-Demirkan⁸, Atilla Dogan¹ and Sinan Saracli⁹

¹Department of Surgery, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Türkiye; ²Department of Anatomy, Faculty of Medicine, Afyonkarahisar Health Sciences University, Afyonkarahisar, Türkiye; ³Department of Food Hygiene, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Türkiye; ⁴Department of Pathology, Faculty of Veterinary Medicine, Bingöl University, Bingöl, Türkiye; ⁵Department of Pathology, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Türkiye; ⁶Department of Medical Pharmacology, Faculty of Medicine, Karabuk University, Karabük, Türkiye; ⁷Department of Pathology, Faculty of Veterinary Sciences, University of Agriculture Faisalabad, Pakistan; ⁸Department of Anatomy, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Türkiye; ⁹Department of Biostatistics and Medical Informatics, Faculty of Medicine, Balikesir University, Balikesir, Türkiye.

*Corresponding author: dr.muhammadnasir@uaf.edu.pk

Abstract

This study was conducted to evaluate the possible anti-tumorigenic effects of Anatolian water buffalo and cow yogurts supplemented with Lactobacillus plantarum or Lactobacillus rhamnosus, and kefir on the development colon cancer (CC) in the mouse model induced with 1,2-dimethylhydrazine (DMH). A total of 440, eight weeks-old, BALB/c male mice were used in the experiment. Mice were allocated into five (05) main groups and further 22 subgroups (20 mice/group). Animals were administered DMH subcutaneously @ 20mg/kg body weight on weekly basis for 12 weeks. In experimental groups, dairy products were given either before DMH administration, simultaneously or after the tumor formation. Tumors were evaluated by the intensity of proliferating cell nuclear antigen, apoptosis and p53 status. Anatolian water buffalo yogurt supplemented with L. rhamnosus showed statistically significant (P<0.05) antitumorigenic effects than the other dairy products used (Anatolian water buffalo yogurt supplemented with L. plantarum, cow yogurt supplemented with L. plantarum or L. rhamnosus and kefir). Hence it was concluded that timely consumption of dairy products (buffalo or cow yogurt supplemented with probiotics, or kefir) have the potential either to prevent or decrease the malignancy of CC.

To Cite This Article: Demirkan I, Ertekin T, Korkmaz M, Pamuk S, Yuksel H, Bozkurt MF, Ozdemir M, Bhaya MN, Cevik-Demirkan A, Dogan A and Saracli S, 2025. Comparison of the anti-tumoral effects of kefir with buffalo and cow yogurt on 1,2-dimethylhydrazine-induced colon cancer in mice. Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2025.291

Abstract

ISSN 0253-8318 (Print)ISSN 2074-7764 (Online)

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)