Avian coccidiosis is one of the major parasitic and economically important diseases affecting the poultry industry, worldwide. Due to drug resistance against Eimeria parasite, alternative therapeutic agents like botanicals may offer a novel, lucrative and cost-effective treatment. Thus, this study aims to evaluate the effectiveness and potential of Pinus radiata extract (PRE) in the treatment of coccidiosis. An In vivo trial was conducted to evaluate anticoccidial potential of PRE.  To this end, 105 (day old) broiler chicks were randomly assigned in seven equal groups (A, B, C, D, E, F and G). At one week of age, all groups except group G, received an oral infection with 50,000 sporulated oocysts of mixed Eimeria parasite.  At 10^th day, Group A, B and C were orally treated with PRE with the dose of 100, 200 and 300mg/kg, respectively. Group D was treated with Vitamin-E. Group E was treated with Baycox^® in drinking water and kept as infected positive control. Group F and G worked as untreated negative control and normal control groups, respectively. Anticoccidial activity was evaluated by oocysts per gram (OPG), fecal score, lesion score, mortality rate and FCR. Hematological parameters such as RBCs, WBCs, hemoglobin and packed cell volume were also determined by using standard protocols. Serum biochemistry (ALT, AST, Urea) was determined by using imported kits. All the collected data was statistically analyzed by ANOVA. The results of in vivo trial showed that PRE showed significant therapeutic effects (P˂0.05) against experimental Eimeria infection by reduction in OPG, lesion and oocysts score in infected chicks. PRE also improved the hematological profile (RBCs, WBCs, and Hb), FCR of chicks and results were significantly different to infected non-medicated control group (P˂0.05). Furthermore, PRE reduced mortality rate and improved organ weight of infected chicks. This study demonstrated that PRE may serve as a sustainable and cost-effective alternative to synthetic anticoccidial drugs, helping to minimize drug resistance and residues in food products while promoting safer poultry production.

To Cite This Article: Abbas A, Manzoor Z, Seyidov M, Rustamli Y, Xubio W, Shoaib M, Husssain K, Raza MA, Sugiharto S, Hailan WA and Mares MM 2025. In vivo anticoccidial, growth promoting and biochemical effects of pinus radiata extract against experimental coccidiosis in broiler chickens. Pak Vet J, 45(3): 1383-1388. http://dx.doi.org/10.29261/pakvetj/2025.268