The present study evaluated the potency of Moringa oleifera leaves extract against testicular dysfunction caused by chronic exposure to nanosilver and sodium arsenite. Sixty-three male rats were divided into seven groups (n=9) each. Group 1 served as the control. Animals of groups 2 and 3 were administered with low (2g/kg body weight) and high (3g/kg BW) dosages of Moringa extract, respectively. Groups 4 and 5 rats received combined low (100 mg/kg BW and 10.25mg/kg BW) and combined high doses (150 mg/kg BW and 16.4 mg/kg BW) of nanosilver and arsenite, respectively. Animals in groups 6 and 7 received combined (low and high) doses of nanosilver, arsenite, and Moringa extract daily and orally for 3 months, as previously described. Three animals from each group were dissected to collect blood and tissue samples at 4, 8 and 12 weeks. Exposure to nanosilver and arsenite at low and high doses resulted in decreased testis weight, a substantial decline in FSH and testosterone activity, but a considerable increase in LH levels. Histochemical analysis revealed reduced activities of antioxidant enzymes (GPx, GST, GSH, CAT and SOD) and elevated MDA levels. Histological examination presented vacuolation, hemorrhage, interstitial tissue edema, and seminiferous tubular deterioration. Administration of Moringa extract improved reproductive hormonal concentrations, reduced MDA, increased antioxidant enzymes activities, and partially mitigated histological alterations in a dose-dependent manner over three months of therapy. Moringa oleifera extract has proven effective as a detoxification strategy and serves as a natural, accessible intervention capable of alleviating the toxic effects of contaminants.

To Cite This Article: Bashir F, Sharif S, Manzoor F, Sattar A and Naz S 2025. Potency of Moringa Leaves Extract against Testicular Dysfunction Induced by Nanosilver and Sodium Arsenite in Mammalian Model. Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2025.231