Diosmetin, is a bioactive flavonoid component of numerous traditional Chinese medicinal herbs such as dandelion and honeysuckle, which has clear anti-inflammatory properties. Based on this efficacy, we examined the anti-inflammatory potential of diosmetin using network pharmacology and in vitro validation. Network pharmacology analysis screened out 41 high-probability anti-inflammatory targets of diosmetin. Enrichment analyses showed that these targets were mainly related to reactive nitrogen metabolism, and the NF-κB cascade served as a dominant hub. In primary bovine mammary epithelial cells (bMECs) model, it was confirmed that diosmetin pre-treatment suppressed IκBα and p65 phosphorylation, blocked NF-κB/iNOS/NO cascade and dimimished the production of IL-1β, TNF-α and IL-6. Our results show that diosmetin exerts anti-inflammatory effect against S. aureus infection in bMECs by targeting the NF-κB/iNOS/NO signaling axis, providing a new mechanistic basis for its potential in clinical veterinary practice

To Cite This Article: Liu J, Wang Z, Chu M, Han Z, Xiao J, Liu R and Bi C, 2026. Diosmetin suppresses s. aureus-triggered inflammation in bovine mammary epithelial cells by modulating the nf-κb/inos/no cascade. Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2026.158