Paraquat is a widely used herbicide known for its high toxicity, posing serious health risks to humans and animals alike. Selenium, a vital trace element, supports the body’s antioxidant defenses. This study explored its protective role against paraquat induced kidney damage, oxidative stress, and blood and biochemical changes in male albino rats. Forty-eight rats were divided into six groups and treated for 28 days: control, selenium only (0.5mg/kg), paraquat alone (10 and 20mg/kg) and paraquat combined with selenium. Blood tests, biochemical analyses, and kidney tissue examinations were conducted to assess the effects. Paraquat exposure significantly disrupted hematological parameters, with marked decreases in Hb, RBC, HCT, MCV, MCH, and MCHC, alongside increases in RDW-CV, WBC, neutrophils lymphocytes, monocytes, eosinophils and platelets counts (P<0.05), indicating anemia and systemic nephrotoxicity. Paraquat also elevated serum urea, uric acid, creatinine, HDL-cholesterol and kidney malondialdehyde levels. While reducing GSH and total antioxidant capacity. Histological analysis confirmed renal tissue damage. Co-administration of Se significantly attenuated these effects, restoring hematological and biochemical markers, reducing oxidative stress, and preserving renal architecture. Paraquat induced kidney damage is strongly linked to oxidative stress. Selenium supplementation effectively mitigated hematological, oxidative stress and renal dysfunction, highlighting its therapeutic potential in counteracting paraquat induced nephrotoxicity.

To Cite This Article: Goraya MU, Shahid R, Ashraf A, Kiran S, Ain Q, Alwutayd KM, Hussain B, Hussain S and Rafique A, 2025. Selenium mitigates Paraquat-Induced hematotoxicity, oxidative stress, biochemical alterations, and nephrotoxicity in male Albino rats. Pak Vet J, 45(3): 1361-1368. http://dx.doi.org/10.29261/pakvetj/2025.267