Endometritis significantly reduces conception rates and milk production in the dairy cows. Serum and glucocorticoid inducible kinase 1 (SGK1) functions as an indispensable mediator of uterine receptivity dynamics during the estrus-mating transition period. It is also implicated in inflammatory processes; SGK1 signaling hubs in the Lipopolysaccharide (LPS) - Toll-like receptors 4 (TLR4) cascades require verification via endometrial epithelial cells specifically transgenic models. In this study, endometritis was first diagnosed by histopathological investigation, and confirmed by increased level of inflammatory cytokines, p65 and c-Jun. SGK1 was downregulated in endometritis tissues. We isolated bovine endometrial epithelial cells (bEECs) and investigated SGK1's role in inflammation by overexpressing or knocking it down, combined with transcriptome and microRNA (miRNA) sequencing in SGK1-overexpressing cells. Our results indicated that the upregulation of SGK1 reduced phospho-modification of NF-κB/JNK subunits, resulting in a reduction of IL-1β, IL-6 and TNF-α. miRNA-143 targeting transforming growth factor-β-activated kinase 1 (TAK1) was identified through mRNA and miRNA sequencing analysis, and subsequently validated in cells via firefly-Renilla luciferase assay. All of SGK1 expression, TAK1 knockdown, and miRNA-143 mimics significantly decreased TAK1 and inflammatory cytokines. Our results demonstrated that SGK1 inhibited the inflammatory response in the endometrium of dairy cows by targeting miRNA-143, which in turn inhibited TAK1.

To Cite This Article: Feng W, Zhou H, Umar T, Chen N, Zhang J, Liu W, Li L and Deng G, 2026. SGK1 suppresses lps-induced inflammatory responses in bovine endometrium via mir-143/tak1 axis. Pak Vet J, 46(2): 382-391. http://dx.doi.org/10.29261/pakvetj/2026.027