The study was conducted on dose optimization of zeolite and its effect on aflatoxin B1 detoxification, nutrients intake and digestibility and dairy performance of Nili Ravi buffaloes. A total of 20 Nili Ravi buffaloes with 500±30kg BW at same milking stage and identical age were distributed randomly into 5 groups (r= 4) fed on aflatoxin B1 (AFB1) and different doses of zeolite powder in total mixed ration (TMR). Diet A (TMR – Control), Diet B (TMR+25μg AFB1/kg feed), Diet C (TMR+25μg AFB1+10g zeolite powder/kg feed), Diet D (TMR+25μg AFB1+15g zeolite powder/kg feed), Diet E (TMR+25μg AFB1+20g zeolite powder/kg feed. The results showed that binding capacity of zeolite for AFB1 was significantly (P<0.05) higher (87.4%) at 15g supplementation as compared to 10 and 20g supplementation in faeces while it was 83.3% in urine. Higher DMI (14.5kg/animal/day) and nutrients digestibility (%) were observed at 15g zeolite supplementation. Blood profile (mg/dL) significantly (P<0.05) increased while HDL and total cholesterol decreased at 15g zeolite supplementation. Significantly (P<0.05) improved milk yield (13.3L) and composition and milk fatty acids profile were found at 15g zeolite supplementation. Carry-over rate of AFB1 to AFM1 in milk was significantly P<0.05) lower (0.14%) at 15g zeolite supplementation. It was concluded that lower carry-over rate of aflatoxin M1 in milk and improved dairy performance of Nili Ravi buffaloes are attributed to dietary supplementation of zeolite at 15g/kg feed.

To Cite This Article: Khaliq A, Mobashar M, Anwar F and Ahmed N, 2026. Dose optimization of zeolite and its effect on aflatoxin detoxification, dry matter intake, nutrients digestibility, production performance and milk fatty acids profile of Nili Ravi buffaloes. Pak Vet J, 46(6): 1603-1610. http://dx.doi.org/10.29261/pakvetj/2026.152