Avian infectious bronchitis virus (IBV) is a highly infectious pathogen, which seriously threatens the global poultry industry. Traditional Chinese Medicine (TCM) has shown some potential in the fight against IBV infection, but its application in the veterinary field is limited by the lack of systematic and massive data support. Based on the TCM inheritance computing platform, this study integrated 34,324 TCM prescriptions, 7,505 Chinese patent medicines and 4,207 platform-specific formulas, screened 336 candidate formulas consistent with IBV clinical phenotypes and obtained 5 core formulas through association rule and clustering analyses. The therapeutic effects of the IBV-infected yellow-feathered broilers were evaluated. The TCM compound had time- and group-dependent anti-IBV effects. TCM-1 performed the best in alleviating clinical symptoms and restoring growth; TCM-1/2/5 alleviated symptoms on the 14^th day and TCM-2/3 significantly promoted weight gain. These formulations protected the thymus and bursa of Fabricius but did not affect the spleen index. Different formulations reduced the viral load in a time- and tissue-dependent manner. TCM-2/3 significantly increased antibody levels, and all formulations balanced inflammatory cytokines and oxidative stress better than ribavirin. TCM-5 showed the best tracheal ciliary protection effect, while TCM-4 had the poorest overall efficacy. This study identified the TCM formula pattern for IBV using a data-mining approach previously established in human medicine and verified their therapeutic potential in a chicken model. The observed effects were associated with immunomodulation, antioxidation, and anti-inflammation. Although the methodology is not entirely novel in human TCM, its application to avian IBV with multi-dimensional in-vivo validation represents an advance in veterinary phytomedicine. This study provides evidence-based candidates for the clinical prevention and treatment of infectious bronchitis in chickens. This study provides evidence-based candidates for the clinical prevention and treatment of infectious bronchitis in chickens.

To Cite This Article: Liu H, Wei X, Pan S, Wang C, Mu W, Momenah MA, Mostafa YS, Suksawat F, Si H, Yang L and Xiang B, 2026. Mining anti-infectious bronchitis virus medication rules and verifying formula efficacy via the tcm inheritance computing platform. Pak Vet J, 46(5): 1092-1109. http://dx.doi.org/10.29261/pakvetj/2026.099