Brucellosis is a major zoonotic disease, causing significant losses in livestock production, especially in low- and middle-income countries. In Pakistan, epidemiological information on bovine brucellosis is scattered, preventing effective disease control. In this study, the seroprevalence of bovine brucellosis was determined, and the associated risk factors were identified in Faisalabad Division, Punjab, Pakistan. The study was designed as a cross-sectional study, with a sample size of 1,000 collected from four districts: Faisalabad, Toba Tek Singh, Jhang, and Chiniot. Rose Bengal Plate Test (RBPT), Serum Agglutination Test (SAT), and indirect ELISA (iELISA) were used to detect anti-Brucella antibodies in serum. Epidemiological data were collected and analyzed using bivariate analysis and multivariable analysis. Seropositivity was 17.90% by RBPT, 16.30% by SAT and 14.70% by iELISA. Inter-test agreement was highest between RBPT & SAT (95.8%), followed by SAT & iELISA (93.4%) and RBPT & iELISA (92.4%). Multivariable analysis using generalized linear mixed-effects models identified larger herd size, older age (>8 years), male sex, pregnancy status, and repeat breeding history as significant independent risk factors for seropositivity (P<0.05). These findings confirm that bovine brucellosis is endemic in Faisalabad Division and highlight the need for active surveillance, strategic vaccination, and improved farm biosecurity.

To Cite This Article: Taimoor M, Ahmad MUD, Hameed H, Shirwany ASAK, Ain QU, Noor H, Ramzan M, Awais MM, Hussain S, Muneer MA, Aslam MS and Guo K, 2026. Seroprevalence and identification of risk factors associated with bovine brucellosis in Faisalabad Division, Punjab, Pakistan. Pak Vet J, 46(6): 1376-1386. http://dx.doi.org/10.29261/pakvetj/2026.133